Two-photon absorption fluorescence is employed within a microfluidic device to create a three-dimensional chemical concentration map for mixing uniformity characterization. This multiphoton technique images fluorescence intensity directly and provides a simple, rapid, and readily employed route to composition characterization within microfluidic systems.
© 2007 Optical Society of America
Original Manuscript: June 25, 2007
Manuscript Accepted: July 16, 2007
Published: August 22, 2007
Vol. 2, Iss. 10 Virtual Journal for Biomedical Optics
Dawn Schafer, Emily A. Gibson, Wafa Amir, Rebecca Erikson, Jodi Lawrence, Tor Vestad, Jeff Squier, Ralph Jimenez, and David W. M. Marr, "Three-dimensional chemical concentration maps in a microfluidic device using two-photon absorption fluorescence imaging," Opt. Lett. 32, 2568-2570 (2007)