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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 10 — Oct. 31, 2007

Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM)

Jan Huisken and Didier Y. R. Stainier  »View Author Affiliations

Optics Letters, Vol. 32, Issue 17, pp. 2608-2610 (2007)

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Multidirectional selective plane illumination microscopy (mSPIM) reduces absorption and scattering artifacts and provides an evenly illuminated focal plane. mSPIM solves two common problems in light-sheet-based imaging techniques: The shadowing in the excitation path due to absorption in the specimen is eliminated by pivoting the light sheet; the spread of the light sheet by scattering in the sample is compensated by illuminating the sample consecutively from opposing directions. The resulting two images are computationally fused yielding a superior image. The effective light sheet is thinner, and the axial resolution is increased by 2 over single-directional SPIM. The multidirectional illumination proves essential in biological specimens such as millimeter-sized embryos. The performance of mSPIM is demonstrated by the imaging of live zebrafish embryos.

© 2007 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: April 11, 2007
Revised Manuscript: July 21, 2007
Manuscript Accepted: July 25, 2007
Published: August 27, 2007

Virtual Issues
Vol. 2, Iss. 10 Virtual Journal for Biomedical Optics

Jan Huisken and Didier Y. R. Stainier, "Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM)," Opt. Lett. 32, 2608-2610 (2007)

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