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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 11 — Nov. 26, 2007

Multispectral photoacoustic imaging of fluorochromes in small animals

Daniel Razansky, Claudio Vinegoni, and Vasilis Ntziachristos  »View Author Affiliations


Optics Letters, Vol. 32, Issue 19, pp. 2891-2893 (2007)
http://dx.doi.org/10.1364/OL.32.002891


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Abstract

Fluorochromes have become essential reporter molecules in biological research. We show that the depth-resolved distribution of fluorochromes in small animals can be imaged with 25 fmol sensitivity and 150 μ m spatial resolution by means of multispectral photoacoustic imaging. The major advantage of the multispectral approach is the sensitive differentiation of chromophores and fluorochromes of interest based on self-reference measurements, as evidenced in this study by resolving a commonly used fluorochrome (Alexa Fluor 750) in mouse. The suggested method is well suited for enhancing visualization of functional and molecular information in vivo and longitudinally.

© 2007 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: July 3, 2007
Revised Manuscript: August 15, 2007
Manuscript Accepted: August 17, 2007
Published: September 28, 2007

Virtual Issues
Vol. 2, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Daniel Razansky, Claudio Vinegoni, and Vasilis Ntziachristos, "Multispectral photoacoustic imaging of fluorochromes in small animals," Opt. Lett. 32, 2891-2893 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-32-19-2891


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