Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source
Optics Letters, Vol. 32, Issue 23, pp. 3408-3410 (2007)
http://dx.doi.org/10.1364/OL.32.003408
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Abstract
We present a time-gated, optically sectioned, hyperspectral fluorescence lifetime imaging (FLIM) microscope incorporating a tunable supercontinuum excitation source extending into the UV. The system is capable of resolving the excitation spectrum, emission spectrum, and fluorescence decays in an optically sectioned image.
© 2007 Optical Society of America
OCIS Codes
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(180.2520) Microscopy : Fluorescence microscopy
(110.4234) Imaging systems : Multispectral and hyperspectral imaging
ToC Category:
Medical Optics and Biotechnology
History
Original Manuscript: September 28, 2007
Revised Manuscript: October 25, 2007
Manuscript Accepted: October 29, 2007
Published: November 21, 2007
Virtual Issues
Vol. 3, Iss. 1 Virtual Journal for Biomedical Optics
Citation
Dylan M. Owen, Egidijus Auksorius, Hugh B. Manning, Clifford B. Talbot, Pieter A. A. de Beule, Christopher Dunsby, Mark A. A. Neil, and Paul M. W. French, "Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source," Opt. Lett. 32, 3408-3410 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-32-23-3408
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