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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 1 — Jan. 29, 2008

Retinal flow cytometer

C. Alt, I. Veilleux, H. Lee, C. M. Pitsillides, D. Côté, and C. P. Lin  »View Author Affiliations

Optics Letters, Vol. 32, Issue 23, pp. 3450-3452 (2007)

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The in vivo flow cytometer is an instrument capable of continuous, real-time monitoring of fluorescently labeled cells in the circulation without the need to draw blood samples. However, the original system probes a single vessel in the mouse ear; the small sample volume limits the sensitivity of the technique. We describe an in vivo retinal flow cytometer that simultaneously probes five artery–vein pairs in the mouse eye by circularly scanning a small laser spot rapidly around the optic nerve head. We demonstrate that the retinal flow cytometer detects about five times more cells per minute than the original in vivo flow cytometer does in the ear.

© 2007 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1530) Medical optics and biotechnology : Cell analysis
(170.1790) Medical optics and biotechnology : Confocal microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: August 10, 2007
Revised Manuscript: October 19, 2007
Manuscript Accepted: October 26, 2007
Published: November 28, 2007

Virtual Issues
Vol. 3, Iss. 1 Virtual Journal for Biomedical Optics

C. Alt, I. Veilleux, H. Lee, C. M. Pitsillides, D. Côté, and C. P. Lin, "Retinal flow cytometer," Opt. Lett. 32, 3450-3452 (2007)

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  1. J. Novak, I. Georgakoudi, X. Wei, A. Prossin, and C. P. Lin, Opt. Lett. 29, 77 (2004). [CrossRef] [PubMed]
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