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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 1 — Jan. 29, 2008

Tissue refractometry using Hilbert phase microscopy

Niyom Lue, Joerg Bewersdorf, Mark D. Lessard, Kamran Badizadegan, Ramachandra R. Dasari, Michael S. Feld, and Gabriel Popescu  »View Author Affiliations


Optics Letters, Vol. 32, Issue 24, pp. 3522-3524 (2007)
http://dx.doi.org/10.1364/OL.32.003522


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Abstract

We present, for the first time to our knowledge, quantitative phase images associated with unstained 5 μ m thick tissue slices of mouse brain, spleen, and liver. The refractive properties of the tissue are retrieved in terms of the average refractive index and its spatial variation. We find that the average refractive index varies significantly with tissue type, such that the brain is characterized by the lowest value and the liver by the highest. The spatial power spectra of the phase images reveal power law behavior with different exponents for each tissue type. This approach opens a new possibility for stain-free characterization of tissues, where the diagnostic power is provided by the intrinsic refractive properties of the biological structure. We present results obtained for liver tissue affected by a lysosomal storage disease and show that our technique can quantify structural changes during this disease development.

© 2007 Optical Society of America

OCIS Codes
(180.3170) Microscopy : Interference microscopy
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: September 28, 2007
Manuscript Accepted: October 30, 2007
Published: December 10, 2007

Virtual Issues
Vol. 3, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Niyom Lue, Joerg Bewersdorf, Mark D. Lessard, Kamran Badizadegan, Ramachandra R. Dasari, Michael S. Feld, and Gabriel Popescu, "Tissue refractometry using Hilbert phase microscopy," Opt. Lett. 32, 3522-3524 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-32-24-3522


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