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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 5 — May. 17, 2007

Fresnel particle tracing in three dimensions using diffraction phase microscopy

YongKeun Park, Gabriel Popescu, Kamran Badizadegan, Ramachandra R. Dasari, and Michael S. Feld  »View Author Affiliations


Optics Letters, Vol. 32, Issue 7, pp. 811-813 (2007)
http://dx.doi.org/10.1364/OL.32.000811


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Abstract

We have developed a novel experimental technique for tracking small particles in three dimensions with nanometer accuracy. The longitudinal positioning of a micrometer-sized particle is determined by using the Fresnel approximation to describe the transverse distribution of the wavefront that originated in the particle. The method utilizes the high-sensitivity quantitative phase imaging capability of diffraction phase microscopy recently developed in our laboratory. We demonstrate the principle of the technique with experiments on Brownian particles jittering in water both in bulk and in the vicinity of a boundary. The particles are localized in space within an error cube of 20 nm × 20 nm × 20 nm for a 33 Hz acquisition rate and 20 s recording time.

© 2007 Optical Society of America

ToC Category:
Microscopy

History
Original Manuscript: November 2, 2006
Revised Manuscript: January 6, 2007
Manuscript Accepted: January 9, 2007
Published: March 5, 2007

Virtual Issues
Vol. 2, Iss. 5 Virtual Journal for Biomedical Optics

Citation
YongKeun Park, Gabriel Popescu, Kamran Badizadegan, Ramachandra R. Dasari, and Michael S. Feld, "Fresnel particle tracing in three dimensions using diffraction phase microscopy," Opt. Lett. 32, 811-813 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-32-7-811

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