Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Microvascular oxygen quantification using two-photon microscopy

Not Accessible

Your library or personal account may give you access

Abstract

An instrument is demonstrated that is capable of three-dimensional (3D) vasculature imaging and pO2 quantification with high spatial resolution. The instrument combines two-photon (2P) microscopy with phosphorescence quenching to measure pO2. The instrument was demonstrated by performing depth-resolved microvascular pO2 measurements of rat cortical vessels down to 120μm below the surface. 2P excitation of porphyrin was confirmed, and measured pO2 values were consistent with previously published data for normoxic and hyperoxic conditions. The ability to perform 3D pO2 measurements using optical techniques will allow researchers to overcome existing limitations imposed by polarographic electrodes, magnetic resonance techniques, and surface-only pO2 measurement techniques.

© 2008 Optical Society of America

Full Article  |  PDF Article
More Like This
In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure

Yu Wang, Song Hu, Konstantin Maslov, Yu Zhang, Younan Xia, and Lihong V. Wang
Opt. Lett. 36(7) 1029-1031 (2011)

Optical monitoring of oxygen tension in cortical microvessels with confocal microscopy

Mohammad A. Yaseen, Vivek J. Srinivasan, Sava Sakadžić, Weicheng Wu, Svetlana Ruvinskaya, Sergei A. Vinogradov, and David A. Boas
Opt. Express 17(25) 22341-22350 (2009)

Quantitation of cerebral oxygen tension using phasor analysis and phosphorescence lifetime imaging microscopy (PLIM)

Chang Liu, Amanda Chisholm, Buyin Fu, Clover T.-Y. Su, İkbal Şencan, Sava Sakadžić, and Mohammad A. Yaseen
Biomed. Opt. Express 12(7) 4192-4206 (2021)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved