OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 6 — Jun. 17, 2008

Microvascular oxygen quantification using two-photon microscopy

Arnold D. Estrada, Adrien Ponticorvo, Tim N. Ford, and Andrew K. Dunn  »View Author Affiliations

Optics Letters, Vol. 33, Issue 10, pp. 1038-1040 (2008)

View Full Text Article

Enhanced HTML    Acrobat PDF (293 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



An instrument is demonstrated that is capable of three-dimensional (3D) vasculature imaging and pO 2 quantification with high spatial resolution. The instrument combines two-photon (2P) microscopy with phosphorescence quenching to measure pO 2 . The instrument was demonstrated by performing depth-resolved microvascular pO 2 measurements of rat cortical vessels down to 120 μ m below the surface. 2P excitation of porphyrin was confirmed, and measured pO 2 values were consistent with previously published data for normoxic and hyperoxic conditions. The ability to perform 3D pO 2 measurements using optical techniques will allow researchers to overcome existing limitations imposed by polarographic electrodes, magnetic resonance techniques, and surface-only pO 2 measurement techniques.

© 2008 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.1460) Medical optics and biotechnology : Blood gas monitoring
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: November 29, 2007
Revised Manuscript: March 20, 2008
Manuscript Accepted: March 21, 2008
Published: May 2, 2008

Virtual Issues
Vol. 3, Iss. 6 Virtual Journal for Biomedical Optics

Arnold D. Estrada, Adrien Ponticorvo, Tim N. Ford, and Andrew K. Dunn, "Microvascular oxygen quantification using two-photon microscopy," Opt. Lett. 33, 1038-1040 (2008)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. J. M. Vanderkooi and D. F. Wilson, Adv. Exp. Med. Biol. 200, 189 (1986). [PubMed]
  2. J. M. Vanderkooi, G. Maniara, and T. J. Green, J. Biol. Chem. 262, 5476 (1987). [PubMed]
  3. R. P. Briñas, T. Troxler, and R. M. Hochstrasser, J. Am. Chem. Soc. 127, 11851 (2005). [CrossRef] [PubMed]
  4. M. Shibata, S. Ichioka, and J. Ando, J. Appl. Physiol. 91, 321 (2001). [PubMed]
  5. R. D. Shonat and A. C. Kight, Ann. Biomed. Eng. 31, 1084 (2003). [CrossRef] [PubMed]
  6. L. Zheng, A. S. Golub, and R. N. Pittman, Am. J. Physiol. 271, H365 (1996). [PubMed]
  7. R. D. Shonat, K. N. Richmond, and P. C. Johnson, Rev. Sci. Instrum. 66, 5075 (1995). [CrossRef]
  8. E. G. Mik, T. G. V. Leeuwen, and N. J. Raat, J. Appl. Physiol. 97, 1962 (2004). [CrossRef] [PubMed]
  9. D. Kleinfeld and W. Denk, in Imaging Neurons (Cold Spring Harbor Laboratory Press, 1999).
  10. I. Dunphy, S. A. Vinogradov, and D. F. Wilson, Anal. Biochem. 310, 191 (2002). [CrossRef] [PubMed]
  11. R. D. Shonat, E. S. Wachman, and W. Niu, Biophys. J. 73, 1223 (1997). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3

Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited