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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 10 — Sep. 22, 2008

Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging

Tom Robinson, Prashant Valluri, Hugh B. Manning, Dylan M. Owen, Ian Munro, Clifford B. Talbot, Christopher Dunsby, John F. Eccleston, Geoff S. Baldwin, Mark A.A. Neil, Andrew J. de Mello, and Paul M.W. French  »View Author Affiliations


Optics Letters, Vol. 33, Issue 16, pp. 1887-1889 (2008)
http://dx.doi.org/10.1364/OL.33.001887


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Abstract

Fluorescence lifetime imaging (FLIM) is used to quantitatively map the concentration of a small molecule in three dimensions in a microfluidic mixing device. The resulting experimental data are compared with computational fluid-dynamics (CFD) simulations. A line-scanning semiconfocal FLIM microscope allows the full mixing profile to be imaged in a single scan with submicrometer resolution over an arbitrary channel length from the point of confluence. Following experimental and CFD optimization, mixing times down to 1.3 ± 0.4 ms were achieved with the single-layer microfluidic device.

© 2008 Optical Society of America

OCIS Codes
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: April 10, 2008
Revised Manuscript: June 10, 2008
Manuscript Accepted: June 18, 2008
Published: August 11, 2008

Virtual Issues
Vol. 3, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Tom Robinson, Prashant Valluri, Hugh B. Manning, Dylan M. Owen, Ian Munro, Clifford B. Talbot, Christopher Dunsby, John F. Eccleston, Geoff S. Baldwin, Mark A. A. Neil, Andrew J. de Mello, and Paul M. W. French, "Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging," Opt. Lett. 33, 1887-1889 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-33-16-1887

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