Confocal diffraction phase microscopy of live cells
Optics Letters, Vol. 33, Issue 18, pp. 2074-2076 (2008)
http://dx.doi.org/10.1364/OL.33.002074
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Abstract
We present a new quantitative phase microscopy technique, confocal diffraction phase microscopy, which provides quantitative phase measurements from localized sites on a sample with high sensitivity. The technique combines common-path interferometry with confocal microscopy in a transmission geometry. The capability of the technique for static imaging is demonstrated by imaging polystyrene microspheres and live HT29 cells, while dynamic imaging is demonstrated by quantifying the nanometer scale fluctuations of red blood cell membranes.
© 2008 Optical Society of America
OCIS Codes
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.1530) Medical optics and biotechnology : Cell analysis
(180.0180) Microscopy : Microscopy
ToC Category:
Imaging Systems
History
Original Manuscript: May 6, 2008
Revised Manuscript: July 23, 2008
Manuscript Accepted: July 24, 2008
Published: September 9, 2008
Virtual Issues
Vol. 3, Iss. 11 Virtual Journal for Biomedical Optics
Citation
Niyom Lue, Wonshik Choi, Kamran Badizadegan, Ramachandra R. Dasari, Michael S. Feld, and Gabriel Popescu, "Confocal diffraction phase microscopy of live cells," Opt. Lett. 33, 2074-2076 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-33-18-2074
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