Laser-induced fluorescence imaging of subsurface tissue structures with a volume holographic spatial-spectral imaging system
Optics Letters, Vol. 33, Issue 18, pp. 2098-2100 (2008)
http://dx.doi.org/10.1364/OL.33.002098
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Abstract
A three-dimensional imaging system incorporating multiplexed holographic gratings to visualize fluorescence tissue structures is presented. Holographic gratings formed in volume recording materials such as a phenanthrenquinone poly(methyl methacrylate) photopolymer have narrowband angular and spectral transmittance filtering properties that enable obtaining spatial–spectral information within an object. We demonstrate this imaging system’s ability to obtain multiple depth-resolved fluorescence images simultaneously.
© 2008 Optical Society of America
OCIS Codes
(090.2890) Holography : Holographic optical elements
(090.4220) Holography : Multiplex holography
(090.7330) Holography : Volume gratings
(100.6890) Image processing : Three-dimensional image processing
(110.0110) Imaging systems : Imaging systems
ToC Category:
Holography
History
Original Manuscript: June 10, 2008
Revised Manuscript: August 3, 2008
Manuscript Accepted: August 6, 2008
Published: September 11, 2008
Virtual Issues
Vol. 3, Iss. 11 Virtual Journal for Biomedical Optics
Citation
Yuan Luo, Paul J. Gelsinger-Austin, Jonathan M. Watson, George Barbastathis, Jennifer K. Barton, and Raymond K. Kostuk, "Laser-induced fluorescence imaging of subsurface tissue structures with a volume holographic spatial-spectral imaging system," Opt. Lett. 33, 2098-2100 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-33-18-2098
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