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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 11 — Oct. 22, 2008

Hyperspectral in vivo two-photon microscopy of intrinsic contrast

Andrew J. Radosevich, Matthew B. Bouchard, Sean A. Burgess, Brenda R. Chen, and Elizabeth M.C. Hillman  »View Author Affiliations

Optics Letters, Vol. 33, Issue 18, pp. 2164-2166 (2008)

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In vivo two-photon imaging of intrinsic contrast can provide valuable information about structural tissue elements such as collagen and elastin and fluorescent metabolites such as nicotinamide adenine dinucleotide. Yet low signal and overlapping emission spectra can make it difficult to identify and delineate these species in vivo. We present a novel approach that combines excitation scanning with spectrally resolved emission two-photon microscopy, allowing distinct structures to be delineated based on their characteristic spectral fingerprints. The amounts of intrinsic fluorophores present in each voxel can also be evaluated. We demonstrate our method using in vivo imaging of nude mouse skin.

© 2008 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(300.6410) Spectroscopy : Spectroscopy, multiphoton
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: April 21, 2008
Revised Manuscript: June 29, 2008
Manuscript Accepted: July 16, 2008
Published: September 12, 2008

Virtual Issues
Vol. 3, Iss. 11 Virtual Journal for Biomedical Optics

Andrew J. Radosevich, Matthew B. Bouchard, Sean A. Burgess, Brenda R. Chen, and Elizabeth M. C. Hillman, "Hyperspectral in vivo two-photon microscopy of intrinsic contrast," Opt. Lett. 33, 2164-2166 (2008)

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