Hyperspectral in vivo two-photon microscopy of intrinsic contrast
Optics Letters, Vol. 33, Issue 18, pp. 2164-2166 (2008)
http://dx.doi.org/10.1364/OL.33.002164
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Abstract
In vivo two-photon imaging of intrinsic contrast can provide valuable information about structural tissue elements such as collagen and elastin and fluorescent metabolites such as nicotinamide adenine dinucleotide. Yet low signal and overlapping emission spectra can make it difficult to identify and delineate these species in vivo. We present a novel approach that combines excitation scanning with spectrally resolved emission two-photon microscopy, allowing distinct structures to be delineated based on their characteristic spectral fingerprints. The amounts of intrinsic fluorophores present in each voxel can also be evaluated. We demonstrate our method using in vivo imaging of nude mouse skin.
© 2008 Optical Society of America
OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(300.6410) Spectroscopy : Spectroscopy, multiphoton
(180.4315) Microscopy : Nonlinear microscopy
ToC Category:
Spectroscopy
History
Original Manuscript: April 21, 2008
Revised Manuscript: June 29, 2008
Manuscript Accepted: July 16, 2008
Published: September 12, 2008
Virtual Issues
Vol. 3, Iss. 11 Virtual Journal for Biomedical Optics
Citation
Andrew J. Radosevich, Matthew B. Bouchard, Sean A. Burgess, Brenda R. Chen, and Elizabeth M. C. Hillman, "Hyperspectral in vivo two-photon microscopy of intrinsic contrast," Opt. Lett. 33, 2164-2166 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-33-18-2164
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