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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 12 — Dec. 1, 2008

Measurement of subcellular texture by optical Gabor-like filtering with a digital micromirror device

Robert M. Pasternack, Zhen Qian, Jing-Yi Zheng, Dimitris N. Metaxas, Eileen White, and Nada N. Boustany  »View Author Affiliations


Optics Letters, Vol. 33, Issue 19, pp. 2209-2211 (2008)
http://dx.doi.org/10.1364/OL.33.002209


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Abstract

We demonstrate an optical Fourier processing method to quantify object texture arising from subcellular feature orientation within unstained living cells. Using a digital micromirror device as a Fourier spatial filter, we measured cellular responses to two-dimensional optical Gabor-like filters optimized to sense orientation of nonspherical particles, such as mitochondria, with a width around 0.45 μ m . Our method showed significantly rounder structures within apoptosis-defective cells lacking the proapoptotic mitochondrial effectors Bax and Bak, when compared with Bax/Bak expressing cells functional for apoptosis, consistent with reported differences in mitochondrial shape in these cells. By decoupling spatial frequency resolution from image resolution, this method enables rapid analysis of nonspherical submicrometer scatterers in an undersampled large field of view and yields spatially localized morphometric parameters that improve the quantitative assessment of biological function.

© 2008 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.1420) Medical optics and biotechnology : Biology
(170.1530) Medical optics and biotechnology : Cell analysis
(070.2615) Fourier optics and signal processing : Frequency filtering
(070.6120) Fourier optics and signal processing : Spatial light modulators

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: June 24, 2008
Revised Manuscript: August 5, 2008
Manuscript Accepted: August 6, 2008
Published: September 26, 2008

Virtual Issues
Vol. 3, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Robert M. Pasternack, Zhen Qian, Jing-Yi Zheng, Dimitris N. Metaxas, Eileen White, and Nada N. Boustany, "Measurement of subcellular texture by optical Gabor-like filtering with a digital micromirror device," Opt. Lett. 33, 2209-2211 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-33-19-2209


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References

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