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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 2 — Feb. 10, 2009

Single-photon-counting detector for increased sensitivity in two-photon laser scanning microscopy

Richard K.P. Benninger, William J. Ashby, Elisabeth A. Ring, and David W. Piston  »View Author Affiliations


Optics Letters, Vol. 33, Issue 24, pp. 2895-2897 (2008)
http://dx.doi.org/10.1364/OL.33.002895


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Abstract

We present the use and characterization of a photon-counting detector for increased sensitivity at low signal levels in fluorescence laser scanning microscopy (LSM). Conventional LSM photomultiplier tube detectors utilize analog current integration and thus suffer from excessive noise at low signal levels, introduced during current measurement. In this Letter we describe the implementation of a fast single-photon-counting (SPC) detector on a conventional two-photon laser scanning microscope and detail its use in imaging low fluorescence intensities. We show that for a low photon flux, the SPC detector is shot-noise limited and thus provides increased detection sensitivity compared with analog current integration.

© 2008 Optical Society of America

OCIS Codes
(180.1790) Microscopy : Confocal microscopy
(230.5160) Optical devices : Photodetectors
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: September 8, 2008
Manuscript Accepted: October 14, 2008
Published: December 4, 2008

Virtual Issues
Vol. 4, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Richard K. P. Benninger, William J. Ashby, Elisabeth A. Ring, and David W. Piston, "Single-photon-counting detector for increased sensitivity in two-photon laser scanning microscopy," Opt. Lett. 33, 2895-2897 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-33-24-2895


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References

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  8. C. Buehler, K. H. Kim, U. Greuter, N. Schlumpf, and P. T. C. So, J. Fluoresc. 15, 41 (2005). [CrossRef] [PubMed]

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