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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 4 — Apr. 23, 2008

Simultaneous time- and wavelength-resolved fluorescence spectroscopy for near real-time tissue diagnosis

Yinghua Sun, Rui Liu, Daniel S. Elson, Christopher W. Hollars, Javier A. Jo, Jesung Park, Yang Sun, and Laura Marcu  »View Author Affiliations

Optics Letters, Vol. 33, Issue 6, pp. 630-632 (2008)

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A novel fiber-optic-based method for simultaneous time- and wavelength-resolved fluorescence spectroscopy for the rapid diagnosis of diseased tissue is demonstrated. By combining multiple bandpass and dichroic filters (405/40, 460/50, and 550/50) with different lengths of optical fiber (1, 10, and 19 m ) acting as an optical delay this system enables the near real-time acquisition and characterization of time-resolved fluorescence spectra using a single detector and excitation input. The recording of multiple fluorescence response pulses at selected wavelengths can be completed in hundreds of nanoseconds, which provides the capability of a real-time characterization of biological systems.

© 2008 Optical Society of America

OCIS Codes
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: December 19, 2007
Revised Manuscript: February 12, 2008
Manuscript Accepted: February 13, 2008
Published: March 14, 2008

Virtual Issues
Vol. 3, Iss. 4 Virtual Journal for Biomedical Optics

Yinghua Sun, Rui Liu, Daniel S. Elson, Christopher W. Hollars, Javier A. Jo, Jesung Park, Yang Sun, and Laura Marcu, "Simultaneous time- and wavelength-resolved fluorescence spectroscopy for near real-time tissue diagnosis," Opt. Lett. 33, 630-632 (2008)

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  1. D. S. Elson, N. Galletly, C. Talbot, J. Requejo-Isidro, J. McGinty, C. Dunsby, P. M. P. Lanigan, I. Munro, R. K. P. Benninger, P. de Beule, E. Auksorius, L. Hegyi, A. Sandison, A. Wallace, P. Soutter, M. A. A. Neil, J. Lever, G. W. Stamp, and P. M. W. French, in Reviews in Fluorescence, C.D.Geddes and J.R.Lakowicz, eds. (Springer, 2006), p. 1.
  2. J. R. Lakowicz, Principle of Fluorescence Spectroscopy (Springer, 2006). [CrossRef]
  3. T. J. Pfefer, D. Y. Paithankar, J. M. Poneros, K. T. Schomacker, and N. S. Nishioka, Lasers Surg. Med. 32, 10 (2003). [CrossRef] [PubMed]
  4. Q. Y. Fang, T. Papaioannou, J. A. Jo, R. Vaitha, K. Shastry, and L. Marcu, Rev. Sci. Instrum. 75, 151 (2004). [CrossRef]
  5. T. Glanzmann, J. P. Ballini, H. van den Bergh, and G. Wagnieres, Rev. Sci. Instrum. 70, 4067 (1999). [CrossRef]
  6. L. Marcu, M. C. Fishbein, J. M. I. Maarek, and W. S. Grundfest, Arterioscler. Thromb. 21, 1244 (2001). [CrossRef]
  7. J. A. Jo, Q. Fang, T. Papaioannou, J. D. Baker, A. H. Dorafshar, T. Reil, J. H. Qiao, M. C. Fishbein, J. A. Freischlag, and L. Marcu, J. Biomed. Opt. 11, 021004 (2006). [CrossRef] [PubMed]
  8. J. D. Pitts and M. A. Mycek, Rev. Sci. Instrum. 72, 3061 (2001). [CrossRef]
  9. J. Requejo-Isidro, J. McGinty, I. Munro, D. S. Elson, N. P. Galletly, M. J. Lever, M. A. A. Neil, G. W. Stamp, P. M. W. French, P. A. Kellett, J. D. Hares, and A. K. L. Dymoke-Bradshaw, Opt. Lett. 29, 2249 (2004). [CrossRef] [PubMed]
  10. J. Siegel, D. S. Elson, S. E. D. Webb, K. C. B. Lee, A. Vlanclas, G. L. Gambaruto, S. Leveque-Fort, M. J. Lever, P. J. Tadrous, G. W. H. Stamp, A. L. Wallace, A. Sandison, T. F. Watson, F. Alvarez, and P. M. W. French, Appl. Opt. 42, 2995 (2003). [CrossRef] [PubMed]
  11. M. V. Rusalov, S. I. Druzhinin, and B. M. Uzhinov, J. Fluoresc. 14, 193 (2004). [CrossRef] [PubMed]
  12. H. Pal, S. Nad, and M. Kumbhakar, J. Chem. Phys. 119, 443 (2003). [CrossRef]

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