Determination of the three-dimensional orientation of single molecules
Optics Letters, Vol. 33, Issue 9, pp. 1020-1022 (2008)
http://dx.doi.org/10.1364/OL.33.001020
Enhanced HTML 
Acrobat PDF (289 KB)
Abstract
A simple method for real-time determination of the full three-dimensional orientation of the emission dipole of single molecules is presented. Introduction of a π phase step in the back focal plane of the collector lens breaks the inherent symmetry, allowing the longitudinal dipole component to be measured. Experimental tolerances and the consequence of a nonzero bandwidth emission spectrum are also discussed. The scheme also allows for the longitudinal component of an electric field to be found experimentally.
© 2008 Optical Society of America
OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(260.2510) Physical optics : Fluorescence
(300.2140) Spectroscopy : Emission
ToC Category:
Microscopy
History
Original Manuscript: February 1, 2008
Manuscript Accepted: March 31, 2008
Published: April 30, 2008
Virtual Issues
Vol. 3, Iss. 6 Virtual Journal for Biomedical Optics
Citation
Matthew R. Foreman, Carlos Macías Romero, and Peter Török, "Determination of the three-dimensional orientation of single molecules," Opt. Lett. 33, 1020-1022 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-33-9-1020
You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
Related Journal Articles 
- Cavity-mode selection in spontaneous emission from oriented molecules in a microparticle (OL)
- Spectroscopy and Laser Characteristics of Copper-Vapor-Laser Pumped Pyrromethene-556 and Pyrromethene-567 Dye Solutions (AO)
- Radiative absorption, fluorescence, and scattering of a classical dipole near a lossless interface: a unified description (JOSAB)
- Luminescence of dye molecules on oxidized silicon and fluorescence interference contrast microscopy of biomembranes (JOSAB)
- Single-molecule orientations determined by direct emission pattern imaging (JOSAB)
Related Conference Papers
- Quantum efficiency measurements in Nd-doped materials
- Luminescence lifetime measurements in Yb3+-doped KY(WO4)2 and KGd (WO4)2
- Modified reciprocity method in laser crystals spectroscopy
- Characterization of malignant tissue cells using laser induced breakdown spectroscopy
- Controlling Fluorescent Proteins by Manipulating the Local Density of Photonic States
« Previous Article | Next Article »
OSA is a member of 