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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 6 — Jun. 17, 2008

Femtosecond Kerr-gated wide-field fluorescence microscopy

Lars Gundlach and Piotr Piotrowiak  »View Author Affiliations


Optics Letters, Vol. 33, Issue 9, pp. 992-994 (2008)
http://dx.doi.org/10.1364/OL.33.000992


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Abstract

We present a Kerr-gated microscope capable of collecting diffraction-limited 2D fluorescence images with sub- 100 fs time resolution. The concept is based on the insertion of a solid-state optical Kerr gate into a wide-field microscope. In addition to the considerably improved temporal resolution, the wide-field design allows for simultaneous tracking of several objects and ultrafast fluorescence lifetime imaging of doped and heterogeneous surfaces. The ultrafast fluorescence dynamics of gold nanoparticles is presented as an example of the capabilities of the instrument.

© 2008 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(190.3270) Nonlinear optics : Kerr effect
(320.7100) Ultrafast optics : Ultrafast measurements
(160.4236) Materials : Nanomaterials

ToC Category:
Ultrafast Optics

History
Original Manuscript: December 21, 2007
Revised Manuscript: March 24, 2008
Manuscript Accepted: March 25, 2008
Published: April 30, 2008

Virtual Issues
Vol. 3, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Lars Gundlach and Piotr Piotrowiak, "Femtosecond Kerr-gated wide-field fluorescence microscopy," Opt. Lett. 33, 992-994 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-33-9-992

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