We demonstrate enhanced axial confinement in a temporal focusing setup with a shaped spectrum and a narrow emission filter, achieving a reduction of 1 order of magnitude of the out-of-focus background when compared with conventional point-scanning two-photon microscopy. By rejecting the background in the optical domain, our technique circumvents the noise problems common in other background subtraction techniques.
© 2009 Optical Society of America
Original Manuscript: February 11, 2009
Revised Manuscript: April 8, 2009
Manuscript Accepted: April 23, 2009
Published: June 3, 2009
Vol. 4, Iss. 8 Virtual Journal for Biomedical Optics
Michael E. Durst, Adam A. Straub, and Chris Xu, "Enhanced axial confinement of sum-frequency generation in a temporal focusing setup," Opt. Lett. 34, 1786-1788 (2009)