Feasibility of in vivo imaging of fluorescent proteins using lifetime contrast
Optics Letters, Vol. 34, Issue 13, pp. 2066-2068 (2009)
http://dx.doi.org/10.1364/OL.34.002066
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Abstract
We show that fluorescence lifetime is a powerful contrast mechanism that can enhance the whole-body imaging of fluorescent proteins (FPs), in the presence of background tissue autofluorescence (AF). The nonexponential AF decay is characterized from time-domain (TD) measurements on multiple nude mice and separated from the FP fluorescence using a linear fit to a priori basis functions. We illustrate this approach using an orthotopic mouse tumor model of breast adenocarcinoma. We also report that four commonly used FPs show distinct lifetimes, indicating their suitability for in vivo lifetime multiplexing. These results suggest the potential for exploiting fluorescence lifetime for imaging FPs for a variety of whole-body small-animal imaging applications.
© 2009 Optical Society of America
OCIS Codes
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6920) Medical optics and biotechnology : Time-resolved imaging
ToC Category:
Medical Optics and Biotechnology
History
Original Manuscript: April 16, 2009
Revised Manuscript: May 8, 2009
Manuscript Accepted: May 20, 2009
Published: June 30, 2009
Virtual Issues
Vol. 4, Iss. 9 Virtual Journal for Biomedical Optics
Citation
Anand T. N. Kumar, Euiheon Chung, Scott B. Raymond, Jeroen A. J. M. van de Water, Khalid Shah, Dai Fukumura, Rakesh K. Jain, Brian J. Bacskai, and David A. Boas, "Feasibility of in vivo imaging of fluorescent proteins using lifetime contrast," Opt. Lett. 34, 2066-2068 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-34-13-2066
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