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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 9 — Sep. 4, 2009

Scanning microscopy using a short-focal-length Fresnel zone plate

Ethan Schonbrun, Winnie N. Ye, and Kenneth B. Crozier  »View Author Affiliations


Optics Letters, Vol. 34, Issue 14, pp. 2228-2230 (2009)
http://dx.doi.org/10.1364/OL.34.002228


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Abstract

We demonstrate a form of scanning microscopy using a short-focal-length Fresnel zone plate and a low-NA relay telescope. Owing to a focal length of only 5 μ m , the zone plate produces large wavefront tilt and consequently severe vignetting for off-axis illumination. By scanning an optically trapped fluorescent sphere, we measure the vignetted collection region of the zone-plate imaging system. The fluorescence collection efficiency is sharply peaked and has a lateral width of 550 nm , which agrees with numerical simulations.

© 2009 Optical Society of America

OCIS Codes
(080.1010) Geometric optics : Aberrations (global)
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy
(050.1965) Diffraction and gratings : Diffractive lenses

ToC Category:
Microscopy

History
Original Manuscript: March 13, 2009
Revised Manuscript: May 31, 2009
Manuscript Accepted: June 10, 2009
Published: July 14, 2009

Virtual Issues
Vol. 4, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Ethan Schonbrun, Winnie N. Ye, and Kenneth B. Crozier, "Scanning microscopy using a short-focal-length Fresnel zone plate," Opt. Lett. 34, 2228-2230 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-34-14-2228


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