We present multiphoton microendoscopy with a rotational probe and a $1\mu \mathrm{m}$ fiber-based femtosecond laser. The rotational probe is based on a double-clad photonic crystal fiber, a gradient index lens, a microprism, and a rotational microelectronicmechanical system (MEMS) motor. The MEMS motor has a diameter of $2.2\mathrm{mm}$ and can provide 360° full-view rotation. The fiber laser provides ultrashort pulses with a central wavelength at $1.034\mu \mathrm{m}$ and a repetition rate of $50\mathrm{MHz}$ . Second-harmonic-generation images of rat-tail tendon and fish scale are demonstrated with the rotational probe-based multiphoton system.