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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 10 — Oct. 2, 2009

In vivo brain imaging using a portable 2.9 g two-photon microscope based on a microelectromechanical systems scanning mirror

Wibool Piyawattanametha, Eric D. Cocker, Laurie D. Burns, Robert P.J. Barretto, Juergen C. Jung, Hyejun Ra, Olav Solgaard, and Mark J. Schnitzer  »View Author Affiliations


Optics Letters, Vol. 34, Issue 15, pp. 2309-2311 (2009)
http://dx.doi.org/10.1364/OL.34.002309


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Abstract

We present a two-photon microscope that is approximately 2.9 g in mass and 2.0 × 1.9 × 1.1 cm 3 in size and based on a microelectromechanical systems (MEMS) laser-scanning mirror. The microscope has a focusing motor and a micro-optical assembly composed of four gradient refractive index lenses and a dichroic microprism. Fluorescence is captured without the detected emissions reflecting off the MEMS mirror, by use of separate optical fibers for fluorescence collection and delivery of ultrashort excitation pulses. Using this microscope we imaged neocortical microvasculature and tracked the flow of erythrocytes in live mice.

© 2009 Optical Society of America

OCIS Codes
(110.2760) Imaging systems : Gradient-index lenses
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.5810) Medical optics and biotechnology : Scanning microscopy
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: March 19, 2009
Manuscript Accepted: April 29, 2009
Published: July 23, 2009

Virtual Issues
Vol. 4, Iss. 10 Virtual Journal for Biomedical Optics
July 23, 2009 Spotlight on Optics

Citation
Wibool Piyawattanametha, Eric D. Cocker, Laurie D. Burns, Robert P. J. Barretto, Juergen C. Jung, Hyejun Ra, Olav Solgaard, and Mark J. Schnitzer, "In vivo brain imaging using a portable 2.9 g two-photon microscope based on a microelectromechanical systems scanning mirror," Opt. Lett. 34, 2309-2311 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-34-15-2309


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