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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 10 — Oct. 2, 2009

Image-based adaptive optics for two-photon microscopy

Delphine Débarre, Edward J. Botcherby, Tomoko Watanabe, Shankar Srinivas, Martin J. Booth, and Tony Wilson  »View Author Affiliations


Optics Letters, Vol. 34, Issue 16, pp. 2495-2497 (2009)
http://dx.doi.org/10.1364/OL.34.002495


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Abstract

We demonstrate wavefront sensorless aberration correction in a two-photon excited fluorescence microscope. Using analysis of the image-formation process, we have developed an optimized correction scheme permitting image-quality improvement with minimal additional exposure of the sample. We show that, as a result, our correction process induces little photobleaching and significantly improves the quality of images of biological samples. In particular, increased visibility of small structures is demonstrated. Finally, we illustrate the use of this technique on various fresh and fixed biological tissues.

© 2009 Optical Society of America

OCIS Codes
(010.7350) Atmospheric and oceanic optics : Wave-front sensing
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(180.4315) Microscopy : Nonlinear microscopy
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Microscopy

History
Original Manuscript: April 23, 2009
Revised Manuscript: June 15, 2009
Manuscript Accepted: June 21, 2009
Published: August 13, 2009

Virtual Issues
Vol. 4, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Delphine Débarre, Edward J. Botcherby, Tomoko Watanabe, Shankar Srinivas, Martin J. Booth, and Tony Wilson, "Image-based adaptive optics for two-photon microscopy," Opt. Lett. 34, 2495-2497 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-34-16-2495

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Supplementary Material


» Media 1: AVI (4146 KB)     
» Media 2: AVI (15206 KB)     

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