We have developed a multimodal imaging technique by integrating photoacoustic microscopy and spectral-domain optical coherence tomography to provide simultaneous volumetric microscopic imaging of both optical absorption and scattering contrasts in biological tissues. In the integrated system, the two imaging modalities share the same optical scanning and delivery mechanisms after their probing and illumination light beams are combined. By further synchronizing the image acquisitions, the images from the two modalities are intrinsically registered. The capabilities of this novel technique were demonstrated by imaging both the microanatomy and microvasculature in mouse ears in vivo.
© 2009 Optical Society of America
Original Manuscript: April 28, 2009
Revised Manuscript: July 20, 2009
Manuscript Accepted: August 21, 2009
Published: September 24, 2009
Vol. 4, Iss. 12 Virtual Journal for Biomedical Optics
Shuliang Jiao, Zhixing Xie, Hao F. Zhang, and Carmen A. Puliafito, "Simultaneous multimodal imaging with integrated photoacoustic microscopy and optical coherence tomography," Opt. Lett. 34, 2961-2963 (2009)