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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 12 — Nov. 10, 2009

Development of a diffraction imaging flow cytometer

Kenneth M. Jacobs, Jun Q. Lu, and Xin-Hua Hu  »View Author Affiliations

Optics Letters, Vol. 34, Issue 19, pp. 2985-2987 (2009)

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Diffraction images record angle-resolved distribution of scattered light from a particle excited by coherent light and can correlate highly with the 3D morphology of a particle. We present a jet-in-fluid design of flow chamber for acquisition of clear diffraction images in a laminar flow. Diffraction images of polystyrene spheres of different diameters were acquired and found to correlate highly with the calculated ones based on the Mie theory. Fast Fourier transform analysis indicated that the measured images can be used to extract sphere diameter values. These results demonstrate the significant potentials of high-throughput diffraction imaging flow cytometry for extracting 3D morphological features of cells.

© 2009 Optical Society of America

OCIS Codes
(110.1650) Imaging systems : Coherence imaging
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.1530) Medical optics and biotechnology : Cell analysis

ToC Category:
Imaging Systems

Original Manuscript: July 6, 2009
Revised Manuscript: August 18, 2009
Manuscript Accepted: August 23, 2009
Published: September 28, 2009

Virtual Issues
Vol. 4, Iss. 12 Virtual Journal for Biomedical Optics

Kenneth M. Jacobs, Jun Q. Lu, and Xin-Hua Hu, "Development of a diffraction imaging flow cytometer," Opt. Lett. 34, 2985-2987 (2009)

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