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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 12 — Nov. 10, 2009

Adaptive harmonic generation microscopy of mammalian embryos

Alexander Jesacher, Anisha Thayil, Kate Grieve, Delphine Débarre, Tomoko Watanabe, Tony Wilson, Shankar Srinivas, and Martin Booth  »View Author Affiliations


Optics Letters, Vol. 34, Issue 20, pp. 3154-3156 (2009)
http://dx.doi.org/10.1364/OL.34.003154


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Abstract

Adaptive optics is implemented in a harmonic generation microscope using a wavefront sensorless correction scheme. Both the second- and third-harmonic intensity signals are used as the optimization metric. Aberration correction is performed to compensate both system- and specimen-induced aberrations by using an efficient optimization routine based upon Zernike polynomial modes. Images of live mouse embryos show an improved signal level and resolution.

© 2009 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(190.4160) Nonlinear optics : Multiharmonic generation
(180.4315) Microscopy : Nonlinear microscopy
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Microscopy

History
Original Manuscript: July 21, 2008
Revised Manuscript: September 8, 2009
Manuscript Accepted: September 11, 2009
Published: October 9, 2009

Virtual Issues
Vol. 4, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Alexander Jesacher, Anisha Thayil, Kate Grieve, Delphine Débarre, Tomoko Watanabe, Tony Wilson, Shankar Srinivas, and Martin Booth, "Adaptive harmonic generation microscopy of mammalian embryos," Opt. Lett. 34, 3154-3156 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-34-20-3154

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