Dual-cladding photonic crystal fibers (PCFs) with two zero-dispersion points are used to enhance the two-photon excited luminescence (TPL) response from fluorescent protein biomarkers and neuron activity reporters in dye-cell experiments and in in vivo work on transgenic mice and tadpoles. The soliton transmission of ultrashort pulses through a PCF suppresses dispersion-induced temporal pulse spreading, maintaining a high level of field intensity needed for efficient TPL excitation. The soliton self-frequency shift, stabilized against laser power fluctuations by a specific PCF dispersion design, is employed to accurately match the wavelength of the soliton PCF output with the two-photon absorption spectrum of dye or fluorescent protein biomarker molecules, enhancing their TPL response and allowing the laser damage of biotissues to be avoided.
© 2009 Optical Society of America
Original Manuscript: July 31, 2009
Manuscript Accepted: September 22, 2009
Published: October 28, 2009
Vol. 4, Iss. 13 Virtual Journal for Biomedical Optics
Lyubov V. Doronina, Il'ya V. Fedotov, Aleksandr A. Voronin, Olga I. Ivashkina, Marina A. Zots, Konstantin V. Anokhin, Ekaterina Rostova, Andrei B. Fedotov, and Aleksei M. Zheltikov, "Tailoring the soliton output of a photonic crystal fiber for enhanced two-photon excited luminescence response from fluorescent protein biomarkers and neuron activity reporters," Opt. Lett. 34, 3373-3375 (2009)