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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 13 — Dec. 2, 2009

High-dynamic-range quantitative phase imaging with spectral domain phase microscopy

Jun Zhang, Bin Rao, Lingfeng Yu, and Zhongping Chen  »View Author Affiliations


Optics Letters, Vol. 34, Issue 21, pp. 3442-3444 (2009)
http://dx.doi.org/10.1364/OL.34.003442


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Abstract

Phase microscopy for high-dynamic-range quantitative phase-contrast imaging of a transparent phase object was demonstrated. Using a common path Fourier domain optical coherence tomography system, this technique is capable of displacement measurement with a sensitivity of 34 pm . The limitation of 2 π ambiguity restriction was overcome by the use of a phase retrieval approach performed in spectral domain. Two-dimensional quantitative phase imaging of human neonatal dermal keratinocyte cells was demonstrated to evaluate the performance of the system for cell imaging.

© 2009 Optical Society of America

OCIS Codes
(110.4500) Imaging systems : Optical coherence tomography
(170.0180) Medical optics and biotechnology : Microscopy
(180.3170) Microscopy : Interference microscopy
(100.5088) Image processing : Phase unwrapping

ToC Category:
Image Processing

History
Original Manuscript: August 3, 2009
Manuscript Accepted: September 17, 2009
Published: October 30, 2009

Virtual Issues
Vol. 4, Iss. 13 Virtual Journal for Biomedical Optics

Citation
Jun Zhang, Bin Rao, Lingfeng Yu, and Zhongping Chen, "High-dynamic-range quantitative phase imaging with spectral domain phase microscopy," Opt. Lett. 34, 3442-3444 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-34-21-3442

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