Automatic deconvolution of 4Pi-microscopy data with arbitrary phase
Optics Letters, Vol. 34, Issue 22, pp. 3583-3585 (2009)
http://dx.doi.org/10.1364/OL.34.003583
Enhanced HTML 
Acrobat PDF (246 KB)
Abstract
We propose a maximum a posteriori-based method that solves an important practical problem in the deconvolution of 4Pi images by simultaneously delivering an estimate of both the object and the unknown phase. The method was tested in simulations and on data from both test samples and biological specimen. It generates object estimates that are free from interference artifacts and reliably recovers arbitrary relative phases. Based on vectorial focusing theory, our theoretical analysis allowed for a simple and efficient implementation of the algorithm. Taking several 4Pi images at different relative phases of the interfering beams is shown to improve the robustness of the approach.
© 2009 Optical Society of America
OCIS Codes
(110.0180) Imaging systems : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(100.1455) Image processing : Blind deconvolution
ToC Category:
Microscopy
History
Original Manuscript: July 2, 2009
Revised Manuscript: October 6, 2009
Manuscript Accepted: October 9, 2009
Published: November 13, 2009
Virtual Issues
Vol. 4, Iss. 13 Virtual Journal for Biomedical Optics
Citation
Giuseppe Vicidomini, Stefan W. Hell, and Andreas Schönle, "Automatic deconvolution of 4Pi-microscopy data with arbitrary phase," Opt. Lett. 34, 3583-3585 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-34-22-3583
You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
Related Journal Articles 
- Multiple-objective microscopy with three-dimensional resolution near 100 nm and a long working distance (OL)
- Optical scanning holography as a technique for high-resolution three-dimensional biological microscopy (JOSAA)
- Nanosizing of Fluorescent Objects by Spatially Modulated Illumination Microscopy (AO)
- Nanosizing of Fluorescent Objects by Spatially Modulated Illuminated Microscopy: Erratum (AO)
- Gaussian approximations of fluorescence microscope point-spread function models (AO)
Related Conference Papers
- Fluorescent Nanoscope - experience of transferring science to business
- Live 3D Imaging of HIV-1 Transfer through the Virological Synapse
- Live 3D Imaging of HIV-1 Transfer through the Virological Synapse
- Live 3D Imaging of HIV-1 Transfer through the Virological Synapse
- Live 3D Imaging of HIV-1 Transfer through the Virological Synapse
« Previous Article | Next Article »
OSA is a member of 