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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 1 — Jan. 4, 2010

Intravital imaging of amyloid plaques in a transgenic mouse model using optical-resolution photoacoustic microscopy

Song Hu, Ping Yan, Konstantin Maslov, Jin-Moo Lee, and Lihong V. Wang  »View Author Affiliations

Optics Letters, Vol. 34, Issue 24, pp. 3899-3901 (2009)

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We report optical-resolution photoacoustic microscopy (OR-PAM) for in vivo imaging of amyloid plaques in an Alzheimer’s disease mouse model. Validation using conventional fluorescence microscopy and multiphoton microscopy shows that OR-PAM has sufficient sensitivity and spatial resolution to identify amyloid plaques in living brains. In addition, with dual-wavelength OR-PAM, the three-dimensional morphology of amyloid plaques and the surrounding microvasculature are imaged simultaneously through a cranial window without angiographic contrast agents. OR-PAM, capable of providing both exogenous molecular contrast and endogenous hemoglobin contrast, has the potential to serve as a new technology for in vivo microscopic observations of cerebral plaque deposits.

© 2009 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: July 8, 2009
Revised Manuscript: November 10, 2009
Manuscript Accepted: November 10, 2009
Published: December 15, 2009

Virtual Issues
Vol. 5, Iss. 1 Virtual Journal for Biomedical Optics

Song Hu, Ping Yan, Konstantin Maslov, Jin-Moo Lee, and Lihong V. Wang, "Intravital imaging of amyloid plaques in a transgenic mouse model using optical-resolution photoacoustic microscopy," Opt. Lett. 34, 3899-3901 (2009)

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