Photobleaching resistance of stimulated parametric emission in microscopy
Optics Letters, Vol. 34, Issue 3, pp. 304-306 (2009)
http://dx.doi.org/10.1364/OL.34.000304
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Abstract
Photobleaching of organic fluorescent labels is a ubiquitous problem in fluorescence microscopy, limiting imaging capabilities and presenting hurdles to quantitative biophysical measurements. We report here that a nonlinear optical signal from some organic fluorophores persists in the presence of photobleaching. Specifically, a four-wave mixing process that is enhanced by a two-photon absorption resonance in the target fluorophore, termed stimulated parametric emission (SPE), is essentially unaffected by the photobleaching of the fluorophore, for rhodamine 6G and other commercial green and red fluorophores. The stability of the SPE signal, and the ability to image weakly or nonfluorescent chromophores, should make this nonlinear microscopy useful for quantitative biophysical measurements.
© 2009 Optical Society of America
OCIS Codes
(190.4380) Nonlinear optics : Nonlinear optics, four-wave mixing
(190.7110) Nonlinear optics : Ultrafast nonlinear optics
(180.4315) Microscopy : Nonlinear microscopy
(190.4975) Nonlinear optics : Parametric processes
ToC Category:
Microscopy
History
Original Manuscript: October 10, 2008
Revised Manuscript: November 20, 2008
Manuscript Accepted: November 29, 2008
Published: January 26, 2009
Virtual Issues
Vol. 4, Iss. 4 Virtual Journal for Biomedical Optics
Citation
Xuejun Liu, Wolfgang Rudolph, and James L. Thomas, "Photobleaching resistance of stimulated parametric emission in microscopy," Opt. Lett. 34, 304-306 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-34-3-304
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