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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 4 — Apr. 1, 2009

Fast-fluorescence dynamics in nonratiometric calcium indicators

M. Gersbach, D. L. Boiko, C. Niclass, C. C. H. Petersen, and E. Charbon  »View Author Affiliations

Optics Letters, Vol. 34, Issue 3, pp. 362-364 (2009)

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The fluorescence decay of high-affinity nonratiometric Ca 2 + indicator Oregon Green BAPTA-1 (OGB-1) is analyzed with unprecedented temporal resolution in the two-photon excitation regime. A triple exponential decay is shown to best fit the fluorescence dynamics of OGB-1. We provide a model for accurate measurements of the free Ca 2 + concentration and dissociation constants of nonratiometric calcium indicators.

© 2009 Optical Society of America

OCIS Codes
(160.2540) Materials : Fluorescent and luminescent materials
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(180.2520) Microscopy : Fluorescence microscopy
(260.2510) Physical optics : Fluorescence
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence

ToC Category:

Original Manuscript: September 4, 2008
Revised Manuscript: November 10, 2008
Manuscript Accepted: November 25, 2008
Published: January 30, 2009

Virtual Issues
Vol. 4, Iss. 4 Virtual Journal for Biomedical Optics

M. Gersbach, D. L. Boiko, C. Niclass, C. C. H. Petersen, and E. Charbon, "Fast-fluorescence dynamics in nonratiometric calcium indicators," Opt. Lett. 34, 362-364 (2009)

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  1. J. Lakowicz, Principles of Fluorescence Spectroscopy (Plenum, 1999).
  2. K. Suhling, P. M. W. French, and D. Phillips, Photochem. Photobiol. Sci. 4, 13 (2005). [CrossRef]
  3. A. V. Agronskaia, L. Tertoolen, and H. C. Gerritsen, J. Biomed. Opt. 9, 1230 (2004). [CrossRef] [PubMed]
  4. C. D. Wilms, H. Schnidt, and J. Eilers, Cell Calcium 40, 73 (2006). [CrossRef] [PubMed]
  5. C. Niclass, A. Rochas, P. A. Besse, and E. Charbon, IEEE J. Solid-State Circuits 40, 1847 (2005). [CrossRef]
  6. W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990). [CrossRef] [PubMed]
  7. A. Habenicht, J. Hjelm, E. Mukhtar, F. Bergstrom, and L. B. A. Johansson, Chem. Phys. Lett. 354, 367 (2002). [CrossRef]
  8. R. Y. Tsien, Biochemistry 19, 2396 (1980). [CrossRef] [PubMed]
  9. D. Thomas, S. C. Tovey, T. J. Collins, M. D. Bootman, M. J. Berridge, and P. Lipp, Cell Calcium 28, 213 (2000). [CrossRef] [PubMed]
  10. J. R. Lakowicz, H. Szmacinski, and M. L. Johnson, J. Fluoresc. 2, 47 (1992). [CrossRef]
  11. M. Eberhard and P. Erne, Biochem. Biophys. Res. Commun. 180, 209 (1991). [CrossRef] [PubMed]
  12. G. Grynkiewicz, M. Poenie, and R. Y. Tsien, J. Biol. Chem. 260, 3440 (1985). [PubMed]

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