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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 5 — May. 5, 2009

Dual-interference-channel quantitative-phase microscopy of live cell dynamics

Natan T. Shaked, Matthew T. Rinehart, and Adam Wax  »View Author Affiliations


Optics Letters, Vol. 34, Issue 6, pp. 767-769 (2009)
http://dx.doi.org/10.1364/OL.34.000767


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Abstract

We introduce and experimentally demonstrate a fast and accurate method for quantitative imaging of the dynamics of live biological cells. Using a dual-channel interferometric setup, two phase-shifted interferograms of nearly transparent biological samples are acquired in a single digital camera exposure and digitally processed into the phase profile of the sample. Since two interferograms of the same sample are acquired simultaneously, most of the common phase noise is eliminated, enabling the visualization of millisecond-scale dynamic biological phenomena with subnanometer optical path length temporal stability.

© 2009 Optical Society of America

OCIS Codes
(090.2880) Holography : Holographic interferometry
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.3170) Microscopy : Interference microscopy
(090.1995) Holography : Digital holography

ToC Category:
Holography

History
Original Manuscript: December 16, 2008
Manuscript Accepted: January 16, 2009
Published: March 6, 2009

Virtual Issues
Vol. 4, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Natan T. Shaked, Matthew T. Rinehart, and Adam Wax, "Dual-interference-channel quantitative-phase microscopy of live cell dynamics," Opt. Lett. 34, 767-769 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-34-6-767


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