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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 5 — May. 5, 2009

Comparing coherent and spontaneous Raman scattering under biological imaging conditions

Meng Cui, Brandon R. Bachler, and Jennifer P. Ogilvie  »View Author Affiliations


Optics Letters, Vol. 34, Issue 6, pp. 773-775 (2009)
http://dx.doi.org/10.1364/OL.34.000773


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Abstract

We compare imaging based on coherent and spontaneous Raman scattering (SpRS) under conditions relevant for biological imaging. Using a broadband laser source, we perform spectral domain imaging of polystyrene beads using coherent Stokes Raman scattering and SpRS and find comparable signal levels. Short interaction lengths, low molecule number, and low incident power all reduce the advantages available with coherent Raman methods. We present calculations to support our measurements.

© 2009 Optical Society of America

OCIS Codes
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(300.6450) Spectroscopy : Spectroscopy, Raman
(180.4315) Microscopy : Nonlinear microscopy
(180.5655) Microscopy : Raman microscopy

ToC Category:
Microscopy

History
Original Manuscript: December 8, 2008
Revised Manuscript: January 23, 2009
Manuscript Accepted: January 23, 2009
Published: March 9, 2009

Virtual Issues
Vol. 4, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Meng Cui, Brandon R. Bachler, and Jennifer P. Ogilvie, "Comparing coherent and spontaneous Raman scattering under biological imaging conditions," Opt. Lett. 34, 773-775 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-34-6-773

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