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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 6 — May. 26, 2009

Hemodynamic measurements from individual blood cells in early mammalian embryos with Doppler swept source OCT

Irina V. Larina, Steven Ivers, Saba Syed, Mary E. Dickinson, and Kirill V. Larin  »View Author Affiliations


Optics Letters, Vol. 34, Issue 7, pp. 986-988 (2009)
http://dx.doi.org/10.1364/OL.34.000986


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Abstract

The most common and lethal birth defects affect the cardiovascular (CV) system. The mouse is a superior model for identifying and understanding mammalian CV birth defects, but there is a great need for tools that can detect early and subtle deficiencies in cardiac function in mouse embryos. We combined swept source optical coherence tomography (SS-OCT) with live mouse embryo culture protocols to generate structural two-dimensional and three-dimensional imaging and hemodynamic measurements in a live 8.5   day embryo just a few hours after the beginning of a heartbeat. Our data show that individual circulating blood cells can be visualized with structural SS-OCT, and using Doppler SS-OCT the velocity of single moving blood cells were measured during different phases of the heartbeat cycle. These results demonstrate that Doppler SS-OCT is an extremely useful tool for structural and hemodynamic analysis at the earliest stages of mammalian blood circulation.

© 2009 Optical Society of America

OCIS Codes
(110.4500) Imaging systems : Optical coherence tomography
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(280.3340) Remote sensing and sensors : Laser Doppler velocimetry
(180.1655) Microscopy : Coherence tomography

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: November 17, 2008
Revised Manuscript: February 11, 2009
Manuscript Accepted: February 18, 2009
Published: March 20, 2009

Virtual Issues
Vol. 4, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Irina V. Larina, Steven Ivers, Saba Syed, Mary E. Dickinson, and Kirill V. Larin, "Hemodynamic measurements from individual blood cells in early mammalian embryos with Doppler swept source OCT," Opt. Lett. 34, 986-988 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-34-7-986


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