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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 6 — May. 26, 2009

Reconstruction of high-resolution holographic microscopic images

M. Kanka, R. Riesenberg, and H. J. Kreuzer  »View Author Affiliations

Optics Letters, Vol. 34, Issue 8, pp. 1162-1164 (2009)

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In in-line holographic microscopy a pinhole illuminates an object and a CCD-detector directly records the hologram in a pixel-pitch-dependent distance. A rapidly calculating exact reconstruction technique using a reorganized hologram with a low number of pixels, the tile superposition technique, is presented. The algorithm is applied on imaging of a 2 μ m bead cluster, and it is compared with other reconstruction techniques. The high-contrast image corresponds to an NA of 0.7. A full 4   megapixel reconstruction with a resolution approaching the diffraction limit is possible in less than a minute. The technique is a base for high-resolution wide-field imaging by multispot illumination.

© 2009 Optical Society of America

OCIS Codes
(090.0090) Holography : Holography
(090.1760) Holography : Computer holography
(100.3010) Image processing : Image reconstruction techniques
(180.0180) Microscopy : Microscopy

ToC Category:

Original Manuscript: November 21, 2008
Revised Manuscript: January 23, 2009
Manuscript Accepted: March 3, 2009
Published: April 6, 2009

Virtual Issues
Vol. 4, Iss. 6 Virtual Journal for Biomedical Optics

M. Kanka, R. Riesenberg, and H. J. Kreuzer, "Reconstruction of high-resolution holographic microscopic images," Opt. Lett. 34, 1162-1164 (2009)

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