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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 6 — May. 26, 2009

Common-path multimodal optical microscopy

Chandra S. Yelleswarapu, Marla Tipping, Sri-Rajasekhar Kothapalli, Alexey Veraksa, and D. V.G.L.N. Rao  »View Author Affiliations

Optics Letters, Vol. 34, Issue 8, pp. 1243-1245 (2009)

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We have developed a common-path multimodal optical microscopy system that is capable of using a single optical source and a single camera to image amplitude, phase, and fluorescence features of a biological specimen. This is achieved by varying either contrast enhancement filters at the Fourier plane and/or neutral density/fluorescence filters in front of the CCD camera. The feasibility of the technique is demonstrated by obtaining brightfield, fluorescence, phase-contrast, spatially filtered, brightfield + fluorescence , phase + fluorescence , and edge- enhanced + fluorescence images of the same Drosophila embryo without the need for image registration and fusion. This comprehensive microscope has the capability of providing both structural and functional information and may be used for applications such as studying live-cell dynamics and in high throughput microscopy and automated microscopy.

© 2009 Optical Society of America

OCIS Codes
(070.0070) Fourier optics and signal processing : Fourier optics and signal processing
(070.6110) Fourier optics and signal processing : Spatial filtering
(110.0180) Imaging systems : Microscopy
(110.4190) Imaging systems : Multiple imaging
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(180.0180) Microscopy : Microscopy

ToC Category:

Original Manuscript: November 20, 2008
Revised Manuscript: March 6, 2009
Manuscript Accepted: March 16, 2009
Published: April 10, 2009

Virtual Issues
Vol. 4, Iss. 6 Virtual Journal for Biomedical Optics

Chandra S. Yelleswarapu, Marla Tipping, Sri-Rajasekhar Kothapalli, Alexey Veraksa, and D. V. G. L. N. Rao, "Common-path multimodal optical microscopy," Opt. Lett. 34, 1243-1245 (2009)

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