An optical configuration for digital holographic microscopy is presented. Digital off-axis holograms are recorded by using a single-cube beam splitter in a nonconventional configuration so as to both split and combine a diverging spherical wavefront emerging from a microscope objective. When a plane numerical reference wavefront is used for the reconstruction of the recorded digital hologram, the phase curvature introduced by the microscope objective together with the illuminating wave to the object wave can be physically compensated.
© 2009 Optical Society of America
Original Manuscript: December 10, 2008
Revised Manuscript: February 13, 2009
Manuscript Accepted: March 10, 2009
Published: April 14, 2009
Vol. 4, Iss. 6 Virtual Journal for Biomedical Optics
Qu Weijuan, Yu Yingjie, Chee Oi Choo, and Anand Asundi, "Digital holographic microscopy with physical phase compensation," Opt. Lett. 34, 1276-1278 (2009)