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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 2 — Jan. 21, 2010

Autoconfocal transmission microscopy based on two-photon-induced photocurrent of Si photodiodes

Chulmin Joo, Chun Zhan, Qing Li, and Siavash Yazdanfar  »View Author Affiliations


Optics Letters, Vol. 35, Issue 1, pp. 67-69 (2010)
http://dx.doi.org/10.1364/OL.35.000067


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Abstract

We describe a simple, self-aligned confocal transmission microscopy technique based on two-photon-induced photocurrents of silicon photodiodes. Silicon detectors produce photocurrents in quadratic dependence on incident intensity under the pulsed illumination of light with wavelengths longer than 1.2 μ m . We exploit this nonlinear process to reject out-of-focus background and perform depth-sectioning microscopic imaging. We demonstrate a comparable background rejection capability of the technique to linear confocal detection and present three-dimensional imaging in biological specimens.

© 2009 Optical Society of America

OCIS Codes
(180.0180) Microscopy : Microscopy
(180.1790) Microscopy : Confocal microscopy
(180.5810) Microscopy : Scanning microscopy
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: August 14, 2009
Revised Manuscript: November 30, 2009
Manuscript Accepted: December 1, 2009
Published: December 24, 2009

Virtual Issues
Vol. 5, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Chulmin Joo, Chun Zhan, Qing Li, and Siavash Yazdanfar, "Autoconfocal transmission microscopy based on two-photon-induced photocurrent of Si photodiodes," Opt. Lett. 35, 67-69 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-35-1-67

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