Image contrast enhancement in confocal ultramicroscopy
Optics Letters, Vol. 35, Issue 1, pp. 79-81 (2010)
http://dx.doi.org/10.1364/OL.35.000079
Enhanced HTML 
Acrobat PDF (281 KB)
Abstract
Ultramicroscopy allows for the 3D reconstruction of centimeter sized samples with a spatial resolution of several micrometers. Nevertheless, in poorly cleared or very large specimens the images may suffer from blurring and low contrast levels. To address these problems, ultramicroscopy was combined with the principle of confocal microscopy using a slowly rotating Nipkow disk. This configuration was tested by comparing images from mouse hippocampal neurons and mouse liver blood vessels recorded in confocal and conventional mode. It was found that confocality minimizes the background noise and considerably improves the signal-to-noise ratio when applied to ultramicroscopy.
© 2009 Optical Society of America
OCIS Codes
(100.3010) Image processing : Image reconstruction techniques
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(330.6110) Vision, color, and visual optics : Spatial filtering
ToC Category:
Microscopy
History
Original Manuscript: September 14, 2009
Revised Manuscript: November 20, 2009
Manuscript Accepted: November 24, 2009
Published: December 31, 2009
Virtual Issues
Vol. 5, Iss. 2 Virtual Journal for Biomedical Optics
Citation
Stefan Kalchmair, Nina Jährling, Klaus Becker, and Hans-Ulrich Dodt, "Image contrast enhancement in confocal ultramicroscopy," Opt. Lett. 35, 79-81 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-35-1-79
You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
Related Journal Articles 
- Superresolving masks for incoherent high-numerical-aperture scanning microscopy in three dimensions (JOSAA)
- Reduced Effects of Spherical Aberration on Penetration Depth Under Two-Photon Excitation (AO)
- Coherent use of opposing lenses for axial resolution increase. II. Power and limitation of nonlinear image restoration (JOSAA)
- Coherent use of opposing lenses for axial resolution increase in fluorescence microscopy. I. Comparative study of concepts (JOSAA)
- Influence of Refractive-Index Mismatch in High-Resolution Three-Dimensional Confocal Microscopy (AO)
Related Conference Papers
- Fluorescence 3D-microscopy with 100 nm resolution
- Deterministically Polarized Fluorescence from Single Dye Molecules Aligned in Liquid Crystal Host
- 3-D reconstructions from cryo electron microscope images: an ab initio algorithm
- Commercial inverted microscope retrofitted for confocal scanning with multi-wavelength detection, spatially resolved spectroscopy, and fluorescence anisotropy
- Tissue Informatics: High Throughput Tissue Cytometry
« Previous Article | Next Article »
OSA is a member of 