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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 2 — Jan. 21, 2010

Image contrast enhancement in confocal ultramicroscopy

Stefan Kalchmair, Nina Jährling, Klaus Becker, and Hans-Ulrich Dodt  »View Author Affiliations


Optics Letters, Vol. 35, Issue 1, pp. 79-81 (2010)
http://dx.doi.org/10.1364/OL.35.000079


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Abstract

Ultramicroscopy allows for the 3D reconstruction of centimeter sized samples with a spatial resolution of several micrometers. Nevertheless, in poorly cleared or very large specimens the images may suffer from blurring and low contrast levels. To address these problems, ultramicroscopy was combined with the principle of confocal microscopy using a slowly rotating Nipkow disk. This configuration was tested by comparing images from mouse hippocampal neurons and mouse liver blood vessels recorded in confocal and conventional mode. It was found that confocality minimizes the background noise and considerably improves the signal-to-noise ratio when applied to ultramicroscopy.

© 2009 Optical Society of America

OCIS Codes
(100.3010) Image processing : Image reconstruction techniques
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(330.6110) Vision, color, and visual optics : Spatial filtering

ToC Category:
Microscopy

History
Original Manuscript: September 14, 2009
Revised Manuscript: November 20, 2009
Manuscript Accepted: November 24, 2009
Published: December 31, 2009

Virtual Issues
Vol. 5, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Stefan Kalchmair, Nina Jährling, Klaus Becker, and Hans-Ulrich Dodt, "Image contrast enhancement in confocal ultramicroscopy," Opt. Lett. 35, 79-81 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-35-1-79

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