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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 10 — Jul. 19, 2010

Theoretical model of errors in micromirror-based three-dimensional particle tracking

Andrew J. Berglund, Matthew D. McMahon, Jabez J. McClelland, and J. Alexander Liddle  »View Author Affiliations


Optics Letters, Vol. 35, Issue 11, pp. 1905-1907 (2010)
http://dx.doi.org/10.1364/OL.35.001905


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Abstract

Several recently developed particle-tracking and imaging methods have achieved three-dimensional sensitivity through the introduction of angled micromirrors into the observation volume of an optical microscope. We model the imaging response of such devices and show how the direct and reflected images of a fluorescent particle are affected. In particle-tracking applications, asymmetric image degradation manifests itself as systematic tracking errors. Based on our results, we identify strategies for reducing systematic errors to the 10 nm level in practical applications.

OCIS Codes
(180.6900) Microscopy : Three-dimensional microscopy
(220.2740) Optical design and fabrication : Geometric optical design
(350.3950) Other areas of optics : Micro-optics

ToC Category:
Microscopy

History
Original Manuscript: December 4, 2009
Revised Manuscript: March 8, 2010
Manuscript Accepted: March 29, 2010
Published: May 28, 2010

Virtual Issues
Vol. 5, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Andrew J. Berglund, Matthew D. McMahon, Jabez J. McClelland, and J. Alexander Liddle, "Theoretical model of errors in micromirror-based three-dimensional particle tracking," Opt. Lett. 35, 1905-1907 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-35-11-1905

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