We demonstrate the feasibility of estimating hemoglobin oxygen saturation ( $\mathrm{Sa}{\mathrm{O}}_2$ ) from erythrocytes over a visible wavelength band using high-resolution spectroscopic optical coherence tomography (SOCT). We take SOCT spectra from individual cells on in vitro human blood phantoms and calculate $\mathrm{Sa}{\mathrm{O}}_2$ by fitting the spectra to an analytical model. We also use diffusive spectroscopy (DS) measurements, which is validated by direct measurement of oxygen partial pressure, as a comparison. The result shows a reliable linear association between the $\mathrm{Sa}{\mathrm{O}}_2$ derived from SOCT and the DS measurement, indicating this methodology has potential on in vivo applications to evaluate blood oxygenation.

© 2010 Optical Society of America

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

Related Journal Articles

• In vivo detection of exercised-induced ultrastructural changes in genetically-altered murine skeletal muscle using polarization-sensitive optical coherence tomography (OE)
• Optical coherence tomography of cell dynamics in three-dimensional tissue models (OE)
• Diffusive and directional intracellular dynamics measured by field-based dynamic light scattering (OE)
• Full-field optical coherence microscopy for identifying live cancer cells by quantitative measurement of refractive index distribution (OE)
• Single-shot Full-field reflection phase microscopy (OE)

Related Conference Papers

• Discrimination of Keratinocytes and Fibroblasts by Parametric Single-cell Tomography
• Non-invasive single cell tomography on skin cells
• Non-invasive single cell tomography on skin cells
• Non-invasive single cell tomography on skin cells
• Non-invasive single cell tomography on skin cells