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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 11 — Aug. 25, 2010

Wide field-of-view microscope based on holographic focus grid illumination

Jigang Wu, Xiquan Cui, Guoan Zheng, Ying Min Wang, Lap Man Lee, and Changhuei Yang  »View Author Affiliations

Optics Letters, Vol. 35, Issue 13, pp. 2188-2190 (2010)

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We have developed a new microscopy design that can achieve wide field-of-view (FOV) imaging and yet possesses resolution that is comparable to a conventional microscope. In our design, the sample is illuminated by a holographically projected light-spot grid. We acquire images by translating the sample across the grid and detecting the transmissions. We have built a prototype system with an FOV of 6 mm × 5 mm and acquisition time of 2.5 s . The resolution is fundamentally limited by the spot size—our demonstrated average FWHM spot diameter was 0.74 μm . We demonstrate the prototype by imaging a U.S. Air Force target and a lily anther. This technology is scalable and represents a cost-effective way to implement wide FOV microscopy systems.

© 2010 Optical Society of America

OCIS Codes
(090.2890) Holography : Holographic optical elements
(170.0110) Medical optics and biotechnology : Imaging systems
(170.5810) Medical optics and biotechnology : Scanning microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: March 22, 2010
Revised Manuscript: May 19, 2010
Manuscript Accepted: May 29, 2010
Published: June 23, 2010

Virtual Issues
Vol. 5, Iss. 11 Virtual Journal for Biomedical Optics

Jigang Wu, Xiquan Cui, Guoan Zheng, Ying Min Wang, Lap Man Lee, and Changhuei Yang, "Wide field-of-view microscope based on holographic focus grid illumination," Opt. Lett. 35, 2188-2190 (2010)

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