Traditional Normarski differential interference contrast (DIC) microscopy is a very powerful method for imaging nonstained biological samples. However, one of its major limitations is the nonquantitative nature of the imaging. To overcome this problem, we developed a quantitative DIC microscopy method based on off-axis sample self- interference. The digital holography algorithm is applied to obtain quantitative phase gradients in orthogonal directions, which leads to a quantitative phase image through a spiral integration of the phase gradients. This method is practically simple to implement on any standard microscope without stringent requirements on polarization optics. Optical sectioning can be obtained through enlarged illumination NA.
© 2010 Optical Society of America
Original Manuscript: April 19, 2010
Manuscript Accepted: May 20, 2010
Published: July 7, 2010
Vol. 5, Iss. 12 Virtual Journal for Biomedical Optics
Dan Fu, Seungeun Oh, Wonshik Choi, Toyohiko Yamauchi, August Dorn, Zahid Yaqoob, Ramachandra R. Dasari, and Michael S. Feld, "Quantitative DIC microscopy using an off-axis self-interference approach," Opt. Lett. 35, 2370-2372 (2010)