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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 12 — Sep. 30, 2010

High-speed multispectral fluorescence lifetime imaging implementation for in vivo applications

Sebina Shrestha, Brian E. Applegate, Jesung Park, Xudong Xiao, Paritosh Pande, and Javier A. Jo  »View Author Affiliations


Optics Letters, Vol. 35, Issue 15, pp. 2558-2560 (2010)
http://dx.doi.org/10.1364/OL.35.002558


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Abstract

Fluorescence lifetime imaging microscopy (FLIM) offers a noninvasive approach for characterizing the biochemical composition of biological tissue. In recent years, there has been an increasing interest in the application of multispectral FLIM for medical diagnosis. Central to the clinical translation of FLIM technology is the development of robust, fast, and cost-effective FLIM instrumentation suitable for in vivo tissue imaging. Unfortunately, the predominant multispectral FLIM approaches suffer from limitations that impede the development of high-speed instruments for in vivo applications. We present a cost-effective scanning multispectral FLIM implementation capable of achieving pixel rates on the order of tens of kilohertz, which will facilitate the evaluation of FLIM for in vivo applications.

© 2010 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(170.7160) Medical optics and biotechnology : Ultrafast technology

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: April 22, 2010
Revised Manuscript: June 25, 2010
Manuscript Accepted: June 26, 2010
Published: July 22, 2010

Virtual Issues
Vol. 5, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Sebina Shrestha, Brian E. Applegate, Jesung Park, Xudong Xiao, Paritosh Pande, and Javier A. Jo, "High-speed multispectral fluorescence lifetime imaging implementation for in vivo applications," Opt. Lett. 35, 2558-2560 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-35-15-2558

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