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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 12 — Sep. 30, 2010

Simultaneous two-wavelength transmission quantitative phase microscopy with a color camera

Matthew T. Rinehart, Natan T. Shaked, Nathan J. Jenness, Robert L. Clark, and Adam Wax  »View Author Affiliations

Optics Letters, Vol. 35, Issue 15, pp. 2612-2614 (2010)

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We present a quantitative phase microscopy method that uses a Bayer mosaic color camera to simultaneously acquire off-axis interferograms in transmission mode at two distinct wavelengths. Wrapped phase information is processed using a two-wavelength algorithm to extend the range of the optical path delay measurements that can be detected using a single temporal acquisition. We experimentally demonstrate this technique by acquiring the phase profiles of optically clear microstructures without 2 π ambiguities. In addition, the phase noise contribution arising from spectral channel crosstalk on the color camera is quantified.

© 2010 Optical Society of America

OCIS Codes
(090.4220) Holography : Multiplex holography
(180.3170) Microscopy : Interference microscopy
(100.5088) Image processing : Phase unwrapping
(090.5694) Holography : Real-time holography

ToC Category:

Original Manuscript: April 13, 2010
Revised Manuscript: June 11, 2010
Manuscript Accepted: June 24, 2010
Published: July 28, 2010

Virtual Issues
Vol. 5, Iss. 12 Virtual Journal for Biomedical Optics

Matthew T. Rinehart, Natan T. Shaked, Nathan J. Jenness, Robert L. Clark, and Adam Wax, "Simultaneous two-wavelength transmission quantitative phase microscopy with a color camera," Opt. Lett. 35, 2612-2614 (2010)

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