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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 5, Iss. 14 — Nov. 16, 2010

Performance limits on three-dimensional particle localization in photon-limited microscopy

Ginni Grover, Sri Rama Prasanna Pavani, and Rafael Piestun  »View Author Affiliations


Optics Letters, Vol. 35, Issue 19, pp. 3306-3308 (2010)
http://dx.doi.org/10.1364/OL.35.003306


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Abstract

We present the performance limits on three-dimensional (3D) localization accuracy of currently used methods of wide-field superlocalization microscopy. The three methods investigated are double-helix microscopy, astigmatic imaging, and biplane detection. In the shot-noise limit, Cramer–Rao lower bound calculations show that, among these techniques, the double-helix microscope exhibits the best axial and 3D localization accuracy over short as well as long depth-of-field systems. The fundamental advantage of engineered point-spread function systems, like the double-helix, stems from the additional degrees of freedom available to control diffraction in three dimensions over variable regions of interest.

© 2010 Optical Society of America

OCIS Codes
(110.4850) Imaging systems : Optical transfer functions
(180.6900) Microscopy : Three-dimensional microscopy
(110.3055) Imaging systems : Information theoretical analysis

ToC Category:
Microscopy

History
Original Manuscript: February 17, 2010
Revised Manuscript: July 28, 2010
Manuscript Accepted: August 11, 2010
Published: September 30, 2010

Virtual Issues
Vol. 5, Iss. 14 Virtual Journal for Biomedical Optics

Citation
Ginni Grover, Sri Rama Prasanna Pavani, and Rafael Piestun, "Performance limits on three-dimensional particle localization in photon-limited microscopy," Opt. Lett. 35, 3306-3308 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-35-19-3306

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