We propose three-dimensional (3D) optical microscopy using axially distributed image sensing. In the proposed method, the micro-objects are optically magnified and their axially distributed images are recorded by moving the image sensor along a common optical axis. The 3D volumetric images are generated from the recorded axial image set using a computational reconstruction algorithm based on ray backprojection. Preliminary experimental results are presented. To the best of our knowledge, this is the first report on 3D optical microscopy using axially distributed sensing.
© 2010 Optical Society of America
Original Manuscript: June 14, 2010
Revised Manuscript: September 20, 2010
Manuscript Accepted: September 28, 2010
Published: October 26, 2010
Vol. 6, Iss. 1 Virtual Journal for Biomedical Optics
Donghak Shin, Myungjin Cho, and Bahram Javidi, "Three-dimensional optical microscopy using axially distributed image sensing," Opt. Lett. 35, 3646-3648 (2010)