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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 6 — Apr. 8, 2010

Three-dimensional microscopy with single-beam wavefront sensing and reconstruction from speckle fields

Arun Anand and Bahram Javidi  »View Author Affiliations

Optics Letters, Vol. 35, Issue 5, pp. 766-768 (2010)

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Generally, 3D digital holographic microscopy requires the interference of the object wave with a known reference beam under coherent illumination to perform numerical focusing. This configuration may be challenging for some applications, including the use of exotic wavelengths such as x rays, miniaturized instrumentation, etc. Single-beam intensity measurement followed by phase retrieval techniques is attractive for wavefront sensing and reconstruction, including applications with low coherence. We use this method to construct a 3D microscope using volume speckle fields. Transparent phase objects are investigated using this principle. To the best of our knowledge, this is the first report on the application of this principle applied to microscopy.

© 2010 Optical Society of America

OCIS Codes
(030.6140) Coherence and statistical optics : Speckle
(100.5070) Image processing : Phase retrieval
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:

Original Manuscript: January 8, 2010
Revised Manuscript: January 8, 2010
Manuscript Accepted: January 31, 2010
Published: February 26, 2010

Virtual Issues
Vol. 5, Iss. 6 Virtual Journal for Biomedical Optics

Arun Anand and Bahram Javidi, "Three-dimensional microscopy with single-beam wavefront sensing and reconstruction from speckle fields," Opt. Lett. 35, 766-768 (2010)

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